Additive manufacturing (AM) by electron beam melting (EBM) is a technique used to manufacture parts by melting powder metal layer-by-layer with an electron beam in a high vacuum, thereby generating a 3D topology. This paper studies the low-cycle fatigue of Ti-6Al-4V specimens obtained by EBM. Static tests were carried out according to ASTM E8 for a yield stress of 1023 MPa, a fracture stress of 1102 MPa, and a maximum tensile strength of 1130 MPa with a maximum true normal strain at fracture ε max = 9.0% and an elastic modulus of 120 GPa. Then, fatigue tests were conducted at a load inversion rate of R = −1. It was observed that the material exhibited plastic strain softening, which was attributed to the Bauschinger effect. These results were plotted on a strain vs. life (ε−N) curve using the Ong version of the Coffin-Manson rule and the Baumel-Seager and Meggiolaro-Castro rules. The results were compared to forged Ti-6Al-4V alloys. The cyclic stress-strain behavior was described with the Ramberg-Osgood model. Finally, the fracture surface was analyzed using scanning electron microscopy (SEM) to observe the formation of primary cracks. The fracture morphology showed a mixed surface, also known as a "quasi-cleavage", which is characterized by dimples, cleavage facets, extensive primary cracks with broken slipping planes, and a large number of inclusions. This phenomenon caused a possible brittle behavior in the material.